Boris Vojnovic Ph.D.

Advanced Technology Development Group
Development of instrumentation to address specific biological hypotheses, primarily involving imaging and image processing.

Prof Boris Vojnovic

Email 
Tel  +44 (0)1865 617376 / +44 (0)1865 617393 (PA)
PA  Linda Nemerofsky-Birks
Email (PA) 
Contact address  Department of Oncology, University of Oxford, Old Road Campus Research Building, Roosevelt Drive, Headington, Oxford OX3 7DQ
Department  Department of Oncology
College No College

Research Summary

The work of the Group involves the development of instrumentation to address specific biological hypotheses, primarily involving imaging and image processing, using automated systems. The particular strengths of the Group are in the application of physics and engineering concepts to design and improve experimental apparatus, from first principles to successful application.

For more information on our research and a full list of publications, please visit:

http://users.ox.ac.uk/~atdgroup/index.shtml

Collaborators

Group Members

  • Paul R Barber, Senior Scientist
  • Iain Tullis, Postdoctoral Researcher
  • Dr Anthony Kavanagh, Postdoctoral Researcher
  • Dr Pamela Reynolds, Postdoctoral Researcher
  • Lisa Folkes, Joint with P O'Neill
  • Robert G Newman, Higher Scientific Officer (Electronics Laboratory)
  • John Prentice, Workshop Manager
  • Daniel Mendes de Oliveria Martins, DPhil Student
  • Davide Volpi, DPhil Student

Collaborators

    Publications

    Selected Publications:

    Merchant, M.J., Jeynes, J.C.G., Grime, G.W., Palitsin, V., Tullis, I.D.C., Barber, P.R., Vojnovic, B., Webb., R.P., Kirkby, K.J. (2012) ‘A focused scanning vertical nanobeam for charged particle irradiation of living cells with single counted particles’. Rad. Res. 178, 182-190.

    Mandeville, H.C., Ng, Q.S., Daley, F.M., Barber, P.R., Pierce, G., Finch, J., Burke, M., Bell, m.b., Townsend, E.R., Kozarski, R., Vojnovic, B., Hoskin, P.J., and Goh, V. (2012) ‘Operable Non-Small Cell Lung Cancer:  Correlation of Volumetric Helical Dynamic Contrast-Enhanced CT Parameters with Immunohistochemical Markers of Tumor Hypoxia’. Radiology. Available at: http://radiology.rsna.org/content/early/2012/06/13/radiol.12111505

    Wilson, P., Jones, B., Yokoi, T., Hill, M., and Vojnovic, B. (2012) ‘Revisiting the Ultra-High Dose Rate Effect:  Implications for Charged Particle Radiotherapy Using Protons and Light Ions’. The British Journal of Radiology. Available at: http://bjr.journals.org/content/early/2012/04/11/bjr.17827549

    Matthews, D.R., Fruhwirth, G.O, Weitsman, G., Carlin, L.M., Ofo, E., Keppler, M., Barber, P.R., Tullis, I.D.C., Vojnovic, B., Ng, T., Ameer-Beg, S.M. (2012) ‘A Multi-Functional Imaging Approach to High-Content Protein Interaction Screening’ PLoS One 7, e33231

    Rothkamm, K., Crosbie, J.C., Daley, F., Bourne, S., Barber, P.R., Vojnovic, B., Cann, L., Rogers, P A. W. (2012) ‘In situ biological dose mapping estimates the radiation burden delivered to ‘spared’ tissue between synchrotron X-ray microbeam radiotherapy tracks’ PloS One 7, (1) e29853

    Baker, M., Robinson, S.D., Lechertier, T., Barber, P.R., Tavora, B., D’Amico, G., Jones, D.T., Vojnovic, B., and Hodivala-Dilke, K. (2012) ‘Use of the mouse aortic ring assay to study angiogenesis’ Nat. Protocols 7, 89-104

    Waterhouse, B.R., Gijsen, M., Barber, P.R., Tullis, I.D.C., Vojnovic, B., and Kong, A. (2011) ‘Assessment of EGFR/HER2 dimerization by FRET-FLIM utilizing Alexa-conjugated secondary antibodies in relation to targeted therapies in cancers,’ Oncotarget 2, 728-736

    Carlin, L.M., Evans, R., Milewicz, H., Fernandes, L., Matthews, D.R., Perani, M., Levitt, J., Keppler, M.D., Monypenny, J., Coolen, T., Barber, P.R., Vojnovic, B., Suhling, K., Fraternali, F., Ameer-Beg, S., Parker, P. J., Thomas, N.S.B., and Ng, T. (2011) ‘A targeted siRNA screen identifies regulators of Cdc42 activity at the natural killer cell immunological synapse’ Sci. Signal. 4:201, ra81

    Ali, R., Gooding, M., Szilágyi, T., Vojnovic, B., Christlieb, M., Brady, M. (2011) ‘Automatic segmentation of adherent biological cell boundaries and nuclei from brightfield microscopy images’ Machine Vision and Applications, Springer (online); DOI: 10.1007/s00138-011-0337-9

    Fruhwirth, G., Fernandes, L., Weitsman, G., Patel, G., Kelleher, M., Lawler, K., Brock, A., Poland, S.  Matthews, D., Keri, G., Barber, P., Vojnovic, B., Ameer-Beg, S., Coolen, A., Fraternali, F., and Ng, T. (2011) ‘How Förster Resonance Energy Transfer Imaging Improves the Understanding of Protein Interaction Networks in Cancer Biology’ Chem. Phys. Chem. 12:  442-461

    Matthews, D., Carlin, L., Ofo, E., Barber, P., Vojnovic, B., Irving, M., Ng, T. and Ameer-Beg, S. (2010) ‘Time-lapse FRET Microscopy using Fluorescence Anisotropy,’ Journal of Microscopy 237(1):51-62

    Makrogianneli, K., Carlin, L.M., Keppler, M.D., Coolen, A.C., Ameer-Beg, S., Barber, P., Vojnovic, B., and Ng, T.(2009) ‘Integrating Receptor Signal Inputs That Influence Small Rho GTPase Activation Dynamics at the Immunological Synapse,’ Molecular and Cellular  Biology 29, 2997-3006  doi:10.1128/MCB.01008-08

    Barber, P.R., Ameer-Beg, S.M., Gilbey, J., Carlin, L.M., Keppler, M.D., Ng, T.C., Vojnovic, B. (2009)  ‘Multiphoton time-domain FLIM: Practical application to protein-protein interactions using global analysis,’ J.R Soc. Interface 6, S93-S105. (doi:10.1098/rsif.2008.0451.focus)

    Prag, S., Parsons, M., Keppler, M.D., Ameer-Beg, S.M., Barber, P., Hunt, J., Beavil, A.J., Calvert, R. Arpin, M., Vojnovic, B. and Ng, T. (2007) ‘Activated Ezrin Promotes Cell Migration through Recruitment of the GEF Dbl to Lipid Rafts and Preferential Downstream Activation of Cdc42,’ Mol. Bio. Cell, 18, 2935–2948

    Atkin, G., Barber, P.R., Vojnovic, B., Daley, F.M., Glynne-Jones, R., and Wilson, G.D. (2005)‘Correlation of spectral imaging and visual grading for the quantification of thymidylate synthase protein expression in rectal cancer’ Human Pathology 36, 1302-1308

    Matthews, D., Summers, H., Njoh, K., Errington, R., Smith, P., Barber, P., Ameer-Beg, S., and Vojnovic, B. (2006) ‘Technique for measurement of fluorescence lifetime by use of stroboscopic excitation and continuous-wave detection,’ Applied Optics 45, 2115-2123

    Ganesan, S., Ameer-Beg, S.M., Ng, T., Vojnovic, B. and Wouters, S.F. (2006) ‘A dark yellow fluorescent protein (YFP)-based Resonance Energy-Accepting Chromoprotein (REACh) for Förster resonance energy transfer with GFP’ PNAS 103 no. 11, 4089–4094